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M94A3174.TXT
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Document 3174
DOCN M94A3174
TI Latent infection of human T-cell leukemia type I (HTLV-I) in human
monocytic cell (THP-1).
DT 9412
AU Ohara Y; Iwasaki Y; Terunuma H; Tsunoda I; Mizobuchi M; Department of
Neurological Sciences, Tohoku University School of; Medicine, Sendai,
Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0164). Unique
Identifier : AIDSLINE ICA10/94369401
AB OBJECTIVE: Besides CD4-positive lymphocytes, monocyte/macrophage (M/M)
lineage cells have been shown to be infected with HTLV-I. Moreover, in
the rat CNS disease induced by inoculation of HTLV-I carrying lymphoid
cells, cellular infiltrates in the CNS are exclusively mononuclear cells
of M/M lineage. For further elucidation of the role of M/M in HTLV-I
infection, we established HTLV-I infected clones from a human monocytic
cell line (THP-1). METHODS: 1 x 10(5) cells of THP-1 cells were
cocultured with an equal number of MT-2 cells treated with mitomycin C
in RPMI 1640 medium supplemented with 10% fetal calf serum. The
propagating cells were serially passaged twice a week. After about three
months, cells were cloned by limiting dilution. 7 clones with positive
HTLV-I signals were then obtained. The integration of HTLV-I were
examined with both Southern blot and PCR. Expression of viral messages
or antigen was also examined by RT-PCR or flowcytometry. RESULTS AND
DISCUSSION: HTLV-I proviral DNA was monoclonally integrated, and message
of either pX or env was hardly detected even with RT-PCR. However, an
overnight culture of the clone in the supernatant of MT-2 cells induced
the expression of viral messages. Therefore, THP-1 cells are thought to
be latently infected with HTLV-I. And this cloned cell line might be
useful to study the relationship between infected lymphocytes and M/M.
DE Blotting, Southern Cell Line Clone Cells Culture Media DNA,
Viral/ANALYSIS Flow Cytometry Gene Expression Genes, env Human
HTLV-I/GENETICS/*PHYSIOLOGY Mitomycin C/PHARMACOLOGY Monocytes
Polymerase Chain Reaction Proviruses/GENETICS/PHYSIOLOGY RNA,
Messenger/BIOSYNTHESIS *Virus Integration MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).